Target Selection for Diagnostic PCR
Several strategies are available for selecting a genetic target to be amplified so as to detect an infectious disease organism. For example, genes that contain both conserved and variable sequence regions may be targeted. In such a case, specificity may be obtained either at the amplification (primer) or detection (probe) stage. The target may also consist of a virulent gene that is uniquely responsible for distinguishing pathogenic from closely related nonpathogenic strains, types, or species.
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