Linked Linear Amplification
Linked Linear Amplification (LLA) is a new nucleic acid amplification method that uses multiple cycles of primer extension reactions. The presence of nonreplicable elements in LLA primers renders primer extension products unusable as templates for further amplification, leading to linear accumulation of products. Through the use of nested primers, linear reactions can be 'linked', providing total amplification yields comparable to those obtained by PCR. The LLA model predicts (a) that amplification yield will approach that of PCR as the number of primers increases and (b) that the unique composition of LLA products will give lower carryover amplification efficiency compared with PCR. LLA is a robust target amplification method with an advantage over PCR that it is more resistant to false results caused by carryover amplicon contamination.
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