Linear RNA amplification
Incyte Corporation's Linear amplification technology is based on antisense RNA amplification and involves a series of enzymatic reactions resulting in T7-based linear amplification of RNA from small amounts of sample RNA. It can be applied to RNA obtained from small biopsies, mRNA-poor cells and tissues, primary cell culture and laser capture micro-dissection (LCM) samples. Unlike exponential amplification methods such as NASBA and RT-PCR, linear RNA amplification maintains relative ratios of the starting mRNA population (representational amplification). Linear RNA amplification technology is used to increase the levels of RNA expressed in cells to quantities that can be used for obtaining gene expression patterns on DNA microarrays and other related applications. This technology can be used for the development of novel diagnostics that identify gene expression patterns unique to particular diseases or disease subtypes.
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